Assay of antibody to group A streptococcal carbohydrate by enzyme-linked immunosorbent assay

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Assay of antibody to group A streptococcal carbohydrate by enzyme-linked immunosorbent assay.

An indirect enzyme-linked immunosorbent assay system for determination of antibody levels to the group A streptococcal cell wall carbohydrate antigen is described. Optimal conditions for antigen preparation, purification, and conjugation to poly-L-lysine for adequate adsorption to the solid phase are presented. Antibody titers of unknown sera were determined by comparison to known reference sta...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1983

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.18.3.622-627.1983